er t2 creer t2 sequence (Addgene inc)
Addgene inc is a verified supplier 
95
Structured Review
Addgene inc
er t2 creer t2 sequence
Er T2 Creer T2 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+er+t2+creer+t2/pCAG-CreERT2+(Plasmid+%2314797)/bio_rxiv__2025__05__23__655316-104-1-21
Average 95 stars, based on 96 article reviews
Er T2 Creer T2 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+er+t2+creer+t2/pCAG-CreERT2+(Plasmid+%2314797)/bio_rxiv__2025__05__23__655316-104-1-21
Average 95 stars, based on 96 article reviews
er t2 creer t2 sequence - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages"
Article Title: CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages
Journal: bioRxiv
doi: 10.1101/2025.05.23.655316
Figure Legend Snippet: (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused CreER is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.
Techniques Used: Translocation Assay, Negative Control, Expressing, Marker, Comparison
Figure Legend Snippet: (A) Confocal images of liver cryosections from 2-month post-fertilization (mpf) Tg(fabp10a:BB-NTR) zebrafish expressing CreER , ERCreER , or DDCreER , without any 4-hydroxytamoxifen (4-OHT) or trimethoprim (TMP) treatment. Fluorescent markers indicate non-recombined nls-mTagBFP2+, or recombined H2B-mGL+ or mCherry-NTR+ hepatocytes. Scale bar: 20 µm. (B) Bar plot representing Mean ± SD showing quantification of background recombination at 2 mpf based on histological analysis of liver sections from Tg(fabp10a:NTR) zebrafish. Significance determined by two-way ANOVA followed by Tukey’s multiple comparisons test. (C) Flow cytometry-based quantification of background recombination at 3 mpf in Tg(fabp10a:BB-NTR) zebrafish. The proportion of mTagBFP2+, H2B-mGL+, mCherry+, and double-positive (H2B-mGL+/mCherry+) hepatocytes was measured by FACS. Each dot represents one animal and the bar plots represent Mean ± SD. Statistical analysis performed using two-way ANOVA followed by Tukey’s multiple comparisons test.
Techniques Used: Expressing, Flow Cytometry
Related Articles
Transgenic Assay:Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: .. Animals Sox10ER T2 CreER T2 (SECE) transgenic mice The ER T2 CreER T2 fragment was digested directly from Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: BrdU labeled cells were detected using an anti-BrdU primary antibody (1:50 dilution, G3G4, DSHB) and Cy3 conjugated affinipure donkey anti-mouse IgG (H+L) secondary antibody (1:300 dilution, 715-165-150, Jackson ImmunoResearch Laboratories). .. Sox10ER T2 CreER T2 (SECE) transgenic mice The ER T2 CreER T2 fragment was digested directly from Plasmid Preparation:Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: .. Animals Sox10ER T2 CreER T2 (SECE) transgenic mice The ER T2 CreER T2 fragment was digested directly from Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: .. The ER T2 CreER T2 fragment was digested directly from Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Article Title: Compartmentalized versus Global Synaptic Plasticity on Dendrites Controlled by Experience Article Snippet: .. SEP-GluR1, SEP-GluR1(S831A,S845A), SEP-GluR2(R586Q), untagged-GluR2(edited) and SEP-GluR3 from rat were PCR amplified and sub-cloned into an expression vector with a ubiquitous promoter CAG, pCALNL. pCALNL-DsRed and Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: BrdU labeled cells were detected using an anti-BrdU primary antibody (1:50 dilution, G3G4, DSHB) and Cy3 conjugated affinipure donkey anti-mouse IgG (H+L) secondary antibody (1:300 dilution, 715-165-150, Jackson ImmunoResearch Laboratories). .. Sox10ER T2 CreER T2 (SECE) transgenic mice The ER T2 CreER T2 fragment was digested directly from Article Title: CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages Article Snippet: The fabp10a:ER T2 CreER T2 ; cryaa:mCerulean construct [abbreviated as fabp10a:ERCreER] was generated by replacing the CreER T2 in fabp10a:CreER T2 ; cryaa:mCerulean construct (Addgene plasmid # 230044 ; http://n2t.net/addgene:230044 ; RRID:Addgene_230044) with ER T2 CreER T2 using EcoRI/NotI. .. The ER T2 CreER T2 sequence was obtained fom Sequencing:Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: .. Animals Sox10ER T2 CreER T2 (SECE) transgenic mice The ER T2 CreER T2 fragment was digested directly from Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: .. The ER T2 CreER T2 fragment was digested directly from Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Article Title: Sox10ER T2 CreER T2 mice enable tracing of distinct neural crest cells populations Article Snippet: BrdU labeled cells were detected using an anti-BrdU primary antibody (1:50 dilution, G3G4, DSHB) and Cy3 conjugated affinipure donkey anti-mouse IgG (H+L) secondary antibody (1:300 dilution, 715-165-150, Jackson ImmunoResearch Laboratories). .. Sox10ER T2 CreER T2 (SECE) transgenic mice The ER T2 CreER T2 fragment was digested directly from Article Title: CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages Article Snippet: The fabp10a:ER T2 CreER T2 ; cryaa:mCerulean construct [abbreviated as fabp10a:ERCreER] was generated by replacing the CreER T2 in fabp10a:CreER T2 ; cryaa:mCerulean construct (Addgene plasmid # 230044 ; http://n2t.net/addgene:230044 ; RRID:Addgene_230044) with ER T2 CreER T2 using EcoRI/NotI. .. The ER T2 CreER T2 sequence was obtained fom Control:Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Mutagenesis:Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Ligand Binding Assay:Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Amplification:Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Article Title: Compartmentalized versus Global Synaptic Plasticity on Dendrites Controlled by Experience Article Snippet: .. SEP-GluR1, SEP-GluR1(S831A,S845A), SEP-GluR2(R586Q), untagged-GluR2(edited) and SEP-GluR3 from rat were PCR amplified and sub-cloned into an expression vector with a ubiquitous promoter CAG, pCALNL. pCALNL-DsRed and Cloning:Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses Article Snippet: To construct pAAV-CaMKIIα-DIO-mCherry for use as a control vector, pAAV-CaMKIIα-DIO-hM3Dq-mCherry was digested with BamHI-HF (R3136, New England Biolabs, Ipswich, MA) and BmtI-HF (R3658, New England Biolabs) to delete the hM3Dq sequence and self-ligated with a linker and the DNA Ligation Kit Mighty Mix (Takara Bio, Otsu, Japan). pAAV-CaMKIIα-DIO-hM4Di-mCherry was produced by exchanging the hM3Dq-mCherry of pAAV-CaMKIIα-DIO-hM3Dq-mCherry with the hM4Di-mCherry from pAAV-hSyn-DIO-hM4Di-mCherry [a gift from B. Roth; Addgene plasmid no. 44362; http://n2t.net/addgene:50475; Research Resource Identifier (RRID): Addgene_44362] using AscI (R0558, New England Biolabs) and NheI-HF (R3131, New England Biolabs) and DNA Ligation Kit Mighty Mix. .. For construction of pAAV-Fos-ER T2 CreER T2 , in which Cre-recombinase is expressed under the control of the Fos minimal promoter and activated in the presence of TAM, a Fos minimal promoter segment cassette from fos-tTA (a gift from M. Mayford; Addgene plasmid no. 34856; http://n2t.net/addgene:34856; RRID: Addgene_34856) , Cre recombinase fused to a mutant estrogen ligand-binding domain ER T2 CreER T2 cassette from Article Title: Compartmentalized versus Global Synaptic Plasticity on Dendrites Controlled by Experience Article Snippet: .. We thank C. Cepko for pCALNL-DsRed (Addgene 13769) and Polymerase Chain Reaction:Article Title: Compartmentalized versus Global Synaptic Plasticity on Dendrites Controlled by Experience Article Snippet: .. SEP-GluR1, SEP-GluR1(S831A,S845A), SEP-GluR2(R586Q), untagged-GluR2(edited) and SEP-GluR3 from rat were PCR amplified and sub-cloned into an expression vector with a ubiquitous promoter CAG, pCALNL. pCALNL-DsRed and Expressing:Article Title: Compartmentalized versus Global Synaptic Plasticity on Dendrites Controlled by Experience Article Snippet: .. SEP-GluR1, SEP-GluR1(S831A,S845A), SEP-GluR2(R586Q), untagged-GluR2(edited) and SEP-GluR3 from rat were PCR amplified and sub-cloned into an expression vector with a ubiquitous promoter CAG, pCALNL. pCALNL-DsRed and Construct:Article Title: Compartmentalized versus Global Synaptic Plasticity on Dendrites Controlled by Experience Article Snippet: .. We thank C. Cepko for pCALNL-DsRed (Addgene 13769) and |
